Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human & Mouse Total Cyclin D1 Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total Cyclin D1 Detection Kit, 50,000 Assay Points
Electron Microscopy Sciences EMS SmartDoc™ 590nm Band Pass Filter for EtBr
For use with EMS SmartDoc gel imaging system.
Revvity Health Sciences Inc HTRF Phospho-c-RAF (Ser43) Detection Kit, 10,000 Assay Points
HTRF Phospho-c-RAF (Ser43) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF cIAP1 BIR3 Binding Kit - Standard
HTRF cIAP1 BIR3 Binding Kit - Standard
Revvity Health Sciences Inc HTRF Phospho-VAV1 (Tyr174) Detection Kit, Control Lysate
HTRF Phospho-VAV1 (Tyr174) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total Estrogen Receptor α Detection Kit, Detection Kit, Control Lysate
HTRF Human Total Estrogen Receptor α Detection Kit, Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total DDR1 Detection Kit, 500 Assay Points
HTRF Human Total DDR1 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human IL-15 Detection Kit Standard, 3 Vials
HTRF Human IL-15 Detection Kit Standard, 3 Vials
Molecular Devices Microplate Reader, Fluorescence, Dual Monochromator, 250-850 nm
The Gemini XPS microplate reader is a top-reading fluorescence spectrofluorometer that provides flexible excitation and emission selection, sensitive detection, and multiple read modes for microplate assays.
- Dual monochromators for excitation and emission wavelength selection.
- Excitation range 250-850 nm.
- Emission range 360-850 nm.
- Supports 6, 12, 24, 48, 96, and 384-well microplates.
- Read types: endpoint, kinetic, spectral scan, and well scan.
- Xenon flash lamp light source.
- Photomultiplier tube detector.
- Temperature control from ambient +4°C to 45°C.
- Approximate weight 35 lb (16 kg) and dimensions 34 x 42 x 42 cm.