Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Total p21 CiP1 Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Total p21 CiP1 Detection Kit, 500 Assay Points
Molecular Devices Excitation Filter, 360 nm, 35 nm Bandwidth
The Molecular Devices 360 nm fluorescence excitation filter is an accessory for FilterMax series multi-mode microplate readers, providing a narrow excitation band centered at 360 nm for assays that require near-UV excitation.
- Provides 360 nm excitation with approximately 35 nm bandwidth.
- Designed for fluorescence assays on microplate readers and ELISA readers.
- Fits FilterMax series reader filter holders for standard microplate formats.
- Improves signal-to-noise for near-UV-excited fluorophores.
- Simple to install and replace during routine maintenance.
Electron Microscopy Sciences Pipette Tip Universal, 5 - 200 mcL
Pipette tips are low binding to ensure accuracy and precision. The low binding surface is the result of a manufacturing process that binds a perfluorooctyl polymer to polypropylene resin–resulting in an exposed trifluoromethyl surface. This surface reduces the surface energy of liquid and repels any substance so sample retention and binding are minimized.
Not detectable/Non-reactive.
Extracted only by a fluorinated solvent.
Autoclaving will not compromise performance.
Available in convenient hinged racked package.
Tips and racks are fully autoclavable. Natural tips feature reference marks at 10 ul, 50 ul, and 100 u. Low binding surface.
Range: 5 - 200 µl
Molecular Devices Absorbance Filter, 620 nm, 8 nm, FilterMax F3/F5
The Molecular Devices AD 340 photometric absorbance filter provides narrowband optical selection for absorbance measurements at 620 nm and is intended as a replacement/auxiliary filter for compatible multi-mode microplate readers.
- 620 nm center wavelength for absorbance measurements.
- 8 nm bandwidth for narrow spectral selection and improved specificity.
- Absorbance/photometric filter design for optical detection assemblies.
- Optimized for assays measuring absorbance near 620 nm.
- Plug-in replacement to maintain instrument optical performance.
Roosterbio Inc RoosterNourish™-MSC-XF: A standardized, xeno-free bioprocess medium for the rapid expansion of hMSCs
RoosterNourish™-MSC-XF is a translation-friendly, xeno-free (XF) bioprocess medium designed to eliminate media exchanges during rapid expansion of human mesenchymal stem/stromal cells (hMSCs) from multiple tissue sources. This medium supports in vitro expansion of hMSCs in a 2D batch process or in a 3D bioreactor fed-batch process with our RoosterReplenish™-MSC-XF bioreactor feed. RoosterNourish™-MSC-XF has unparalleled productivity (Millions of cells produced per Liters of media consumed) compared to other MSC media on the market. Used alongside our XF RoosterVial™-MSC and following RoosterBio protocols, we guarantee 10X hMSC expansion within 4-6 days. This medium has been used to generate high volumes of hMSC critical raw materials worldwide and has supported top regenerative medicine research, product developers, and translational institutions.Intended Use: Research Use Only
Revvity Health Sciences Inc HTRF Mouse Phospho-STING (Ser365) Detection Kit, 500 Assay Points
HTRF Mouse Phospho-STING (Ser365) Detection Kit, 500 Assay Points
Enterprise Technology Solutions Customizable Index Dividers
Customizable TOC Ready Index Multicolor Tab Dividers 10-Tab 1 to 10 11 x 8 5 White Traditional Color Tabs 6 Sets
Revvity Health Sciences Inc HTRF Human Total HPK1 Detection Kit, 50,000 Assay Points
HTRF Human Total HPK1 Detection Kit, 50,000 Assay Points
Molecular Devices Emission Filter, 710 nm, 50 nm BW, TRF
This optical emission filter is specified at a 710 nm center wavelength with a 50 nm bandwidth and is intended for fluorescence, luminescence, and time-resolved fluorescence (TRF) detection in compatible multi-mode microplate readers.
- Center wavelength 710 nm.
- Bandwidth 50 nm for narrow spectral selection.
- Suitable for fluorescence, luminescence, and TRF assays.
- Accessory filter for compatible multi-mode microplate readers.
- Improves signal-to-noise for near-infrared detection.
Revvity Health Sciences Inc Stainless Steel Beads Bulk, 2.4 mm, 500 g
Stainless Steel Beads Bulk, 2.4 mm, 500 g
Sigma Aldrich Fine Chemicals Biosciences TranscreenerR ADP2 TR fRET
The Transcreener(R) ADP2 TR-fRET Red Assay is a competitive immunoassay for ADP with a far-red time-resolved foumlrster-resonance-energy-transfer (TR-fRET) readout. Because it is highly selective for ADP the assay can be used with any enzyme that converts ATP to ADP regardless of what other substrates are used. Examples of enzymes include protein lipid and carbohydrate kinases ATPases DNA helicases carboxylases and glutamine synthetase. The Transcreener(R) assay is designed specifically for high-throughput screening (HTS) with a single addition mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener(R) HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families. The Transcreener(R) ADP2 TR-fRET Red Assay provides the following benefitsbullnbsp Accommodates ATP concentra
Revvity Health Sciences Inc HTRF CHO HCP Detection Kit, 10,000 Assay Points
HTRF CHO HCP Detection Kit, 10,000 Assay Points